ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF LAFUTIDINE IN TABLET DOSAGE
Keywords:
A simple, sensitive and rapid reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of lafutidinine in tablet dosage form. Hypersil silica, C18 250× 4.6 mm. 5µ. column. was used with a mobile phase containing a mixture 0.02m dihydrogen potassium ortho phosphate,0.02m dipotasium hydrogen ortho mphasphate(pH 6) and Acetonitrile and in the ratio of 30:70. The flow rate was 1.0ml/min and effluents were monitored at 215nm and eluted at 7.75min respectively. Calibration curve was plotted with a range from 27- 81µg/ml. The assay was validated for the parameters like accuracy, precision, robustness and system suitability parameters. The proposed method can be useful in the routine analysis for the determination on lafutidinine in tablet dosage.Abstract
A simple, sensitive and rapid reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of lafutidinine in tablet dosage form. Hypersil silica, C18 250× 4.6 mm. 5µ. column. was used with a mobile phase containing a mixture 0.02m dihydrogen potassium ortho phosphate,0.02m dipotasium hydrogen ortho
mphasphate(pH 6) and Acetonitrile and in the ratio of 30:70. The flow rate was 1.0ml/min and effluents were monitored at 215nm and eluted at 7.75min respectively.
Calibration curve was plotted with a range from 27-81µg/ml. The assay was validated for the parameters like accuracy, precision, robustness and system suitability parameters. The proposed method can be useful in the routine analysis for the determination on lafutidinine in tablet dosage.
, 
