PROTECTIVE EFFECT OF DILLENIA INDICA L. ON DOXORUBICIN INDUCED CARDIOTOXICITY IN RATS

Abstract

Objectives: To investigate the effect of the ethanolic extract of Dillenia indica L. leaves against doxorubicin induced myocardial toxicity in rats. Materials and Methods: Cardiotoxicity was produced by doxorubicin administration (3 mg/kg/week, i.p. for 5 weeks). Ethanolic extract of Dillenia indica L. leaves (250 mg/kg and 500 mg/kg orally) was administered as pretreatment for 30 days. The biomarker enzymes like lactate dehydrogenase (LDH), creatinine kinase (CK), diagnostic enzyme markers like glutamic oxaloacetate transaminase (SGOT) and glutamic pyruvic transaminase (SGPT), and antioxidants such as glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), lipid peroxidase (LP) were monitored. Also membrane bound enzymes like Na+K+ATPase, Ca2+ ATPase and Mg2+ ATPase were monitored. Results: Pretreatment with the ethanolic extract of Dillenia indica L. significantly (P < 0.05) protected the myocardium from the toxic effects of doxorubicin by reducing the elevated level of biomarker and diagnostic enzymes like LDH, CPK, SGOT and SGPT to the normal levels. Ethanolic extract of Dillenia indica L. leaves increased the GSH, SOD and CAT levels and decreased the MDA levels in cardiac tissue. Administration of doxorubicin caused cardiomyopathy associated with an antioxidant deficiency. Conclusion: These results suggest a cardioprotective effect of Dillenia indica L. due to its antioxidant properties