STANDARDIZATION, HPLC ANALYSIS AND ANTIOXIDANT STUDY OF AN AYURVEDIC FORMULATION-DATHRYARISHTAM

Abstract

Standardization assures that products are reliable in terms of quality, efficacy, performance and safety. From the standardization part of view, chemical values of the individual drugs in the finished products can be used as preliminary reference standards for market samples of the single drugs. Dathryarishtam is one of the arishtam referred from ‘Charaka Samhitha’, which is good for anemia, chronic fever and loss of appetite. The aim of this study is to fix the physico-chemical standards of Dathryarishtam and the drugs used in the formulation. Each drug is characterized on the basis of their intrinsic chemical constituent. For the standardization, the extracts are subjected to chemical test, physical studies (ash & extractive values, pH, reducing sugar, alcohol content and etc.) and chromatographical studies (TLC & HPLC). Heavy metal contamination (AAS) also determined in the formulation. Moreover we have qualitatively and quantitatively identified the presents of marker compounds in each herbs used in the formulations and the variations in batch-to-batch preparations. The standardization of herbal drugs and their formulations will be effective when the above-mentioned methods used in combination, each method playing a complementary role. There is a crying need to apply these methods to standardize the herbs and their formulations to find global acceptability. The analyses were carried out in two different batches of our production and averages of the results are kept as reference. We hope our research findings will help the Ayurvedic industry for formulating/marketing standard drug with international acceptance.