NANOSUSPENSION APPROACH OF ACYCLOVIR USING HET-CAM METHOD

Abstract

Topical application of drugs to the eye is the most popular and well-accepted route of administration for the treatment of various eye disorders with various drugs like ACV (Acyclovir), an antiviral drug with a significant and highly specific activity against herpes viruses, particular, herpes simplex keratitis, with its poor water solubility (0.2 mg/ml) in nanoparticle formulation by four different methods are I: Nanoprecipitation method, II: Modified nanoprecipitation method. III: Single emulsification method and IV: Double emulsification method. The prepared nanoparticles were evaluated for particle size, entrapment efficiency, zeta potential and Polydispersity index, 300±25 nm, 66 %, (-)50.3 mv and 0.08 respectively. PLGA (Poly(d,l-lactide-co-glycolide)) nanoparticles may provide a sustained ocular drug release, prolonged ocular residence, enhanced acyclovir bioavailability and ease of application in form of liquid eye drop (nanosuspension).