EFFECT OF LEPIDIUM SATIVUM ON MEMBRANE BOUND PHOSPHATISES (ATPASE) AND HISTOPATHOLOGICAL CHANGE IN RENAL TOXICITY

Abstract

The present study was designed to determine the possible effect of ethanolic extract of Lepidium sativum seeds on
alteration membrane bound phosphatises (ATPase) and histopathology of acute renal failure. The 200 mg/kg and 400
mg/kg ethanolic extract of Lepidium sativum L. seeds was used to against cisplatin (5 mg/kg, i.p.) induced renal toxicity.
The experimental protocol designed as the animals were divided into six groups (n=6) like control, model control, two
protective and two curative groups were received vehicle, cisplatin, cisplatin + extract, and extract + cisplatin
respectively. After 6th days, the rats were sacrificed for quantitative estimation membrane bound phosphatises (ATPase)
and histopathology in kidney tissue. The level of brush border enzymes like Na+ / K+ ATPase, Ca++ ATPase and Mg++ATPase
were decreased significantly in model control and. It was overcome by same extract treatment in curative and protective
groups, and histopathology changes in model control group like hyaline droplets, tubular dilation was observed, it was
cured more in curative groups (200 mg/kg) and protective (400 mg/kg) then the protective (200 mg/kg) and curative
groups (400 mg/kg). Finally it is concluded that the present study data conformed acute renal toxicity induced by
cisplatin due oxidative stress and ethanolic extract of Lepidium sativum L. seeds could contributed protective and curative
effect